Cellulose Acetate (CA) Filtration Membrane

GVS Life Sciences Cellulose Acetate (CA) Filtration Membrane is a supported, hydrophilic membrane that exhibits naturally low protein binding. Composed of cellulose acetate internally supported by an inert polyester web, the resulting membrane has dimensional stability. This provides higher throughputs than competitor offerings and reduces the amount of filter changes needed during filtration of proteinaceous solutions. It is ideal for use in filtration applications where maximal recovery of protein is critical.  

GVS Life Sciences CA Membrane is available in roll widths from 25 mm (1 in) to 30 cm (12 in), as well as in sheets and disks that can be customized to meet your application and size requirements. 

Features & Benefits
Superior strength to withstand aggressive handling or use with automated equipment without breaking or tearing
- Low protein binding (BSA binding capacity of 3.8 μg/cm²) minimizes retention of proteins in solution
- Low extractables ensure tests will be clean with consistent results
- Lot-to-lot consistency ensures consistent flow and diffusion rates for dependable results every time
- Non-lysing of cells prevents contamination of critical solutions
- Passes USP Class VI Toxicity testing, ensuring suitability for use in medical devices
- The materials in this membrane are FDA approved for food contact under the applicable regulations in 21 CFR 

Product Characteristics
- Hydrophilic
- Thermally stable with a maximum operating temperature of 274°F (135°C)
- Autoclavable
- Broad sealing compatibility, including ultrasonic, heat, radio frequency and insert molding

Typical Applications
- Protein and enzyme filtration
- Biological fluid sterilization
- Tissue culture media sterilization
- Cold sterilization
- Wine filtration
- Prefiltration of plasma fractions and vaccines

Specifications 

Polyethersulfone (PES) Filtration Membrane 

GVS Life Sciences Polyethersulfone (PES) Filtration Membrane is naturally hydrophilic, has low extractables and is cast from pure polyethersulfone polymer. This strong, microporous membrane is constructed from a high-temperature polyethersulfone polymer that is acid and base resistant. Its strength and durability are advantageous during usage that involves aggressive handling or automated equipment. It is designed to remove particulates during general filtration, and its low protein and drug binding characteristics make it ideally suited for use in life sciences applications.  

GVS Life Sciences PES Filtration Membrane is available in roll widths from 1.27 cm (0.5 in) to 79 cm (31 in), as well as in cut sheets and disks that can be customized to meet your application and size requirements. 

Features & Benefits
- Low extractables ensure clean results
- Low drug and protein binding maximize sample recovery
- Superior burst strength protects integrity of membrane under high pressures
- Lot-to-lot consistency for reproducible results every time
- Endotoxin and USP Class VI test results ensure membrane is suitable for use in medical devices
The materials in this membrane are FDA approved for food contact under the applicable regulations in 21 CFR

Product Characteristics
- Hydrophilic
- Maximum operating temperature: 266°F (130°C)
- Autoclavable
- Broad sealing compatibility, including ultrasonic, heat, radio frequency and insert molding 

Typical Applications
- Protein and enzyme filtration and sterilization
- Biological fluid filtration and sterilization
- Pharmaceutical sterilization
- Environmental water studies
- Coarse particulate filtration (large pore)
- Clarification and final filtration (small pore) 

Specifications 

Nylon (NY) Membrane    

GVS Life Sciences Nylon (NY) Membrane is a supported, naturally hydrophilic membrane designed to wet out evenly and retain its superior strength during use in general filtration or medical assays. The membrane is internally supported with an inert polyester support web, giving it added dimensional strength and stability that prevents cracking, tearing, curling and breaking. This is advantageous during usage that involves aggressive handling or automated equipment. GVS Life Sciences NY Membrane is produced in filtration and hybridization grades to specifications optimized for those particular applications.    

GVS Life Sciences NY Membrane is available in roll widths of 1.27 cm (0.5 in) to 33 cm (13 in), as well as in cut sheets and disks that can be customized to meet your application and size requirements.  

Features & Benefits
- Superior strength for easy handling
- Lot-to-lot consistency provides reproducible results every time
- Passes USP Class VI toxicity testing, for use in medical devices
- High nucleic acid binding capacity of 350-450 μg/cm2 makes this membrane suitable for molecular biology applications and allows binding of a broad range of nucleic acid fragment sizes (75 bp - 50 kbp)
- Low extractables ensure clean results
- The materials in this membrane are FDA compliant for food contact under the applicable regulations in 21 CFR  

Product Characteristics
- Hydrophilic
- Maximum operating temperature: 356°F (180°C)
- Autoclavable
- Broad sealing compatibility, including ultrasonic, heat, radio frequency and insert molding  

NY Filtration Membrane
Typical Applications
- Sterilization and clarification of aqueous, alkaline and organic solvent solutions
- HPLC sample preparation
- Beverage and pharma processing

Specifications   

Neutral Nylon Molecular Transfer (Blotting) Membrane  

GVS Life Sciences Neutral Nylon Molecular Transfer Membrane is a pure polymer cast on an inert polyester web.  

Typical Applications 
- Southern and Northern blots (nucleic acids)
- Microarrays
- Macroarrays
- Dot/slot blots
- Radiolabeled detection systems
- Non-radiolabeled detection systems
- Colony lifts
- Plaque lifts
- Library screening  

 Specifications    

Reprobing, Positively Charged NY Molecular Transfer (Blotting) Membrane
This NY Molecular Transfer Membrane variation is an inherently charged nylon membrane, specifically designed to allow for numerous reprobings. GVS Life Sciences reprobing, positively charged NY membrane has been tested to provide consistent results through 12 or more reprobings.

Typical Applications
- Radiolabeled and non-radiolabeled detection systems
- Northern and Southern blots (nucleic acids)
- Multiple reprobings
- Alkaline transfers
- DNA fingerprinting
- UV crosslinking

Nitrocellulose (NC) Membrane

GVS Life Sciences Nitrocellulose (NC) Membrane is produced as an unsupported filtration membrane and as a supported or unsupported molecular transfer membrane for bio-analyses. Specifications for each type of NC membrane are optimized for its specific application. NC membranes are naturally hydrophilic with a rapid flow rate and high throughput, which makes them ideal for use in diagnostic and filtration kit manufacturing applications. These membranes are composed of a mixture of inert cellulose nitrate and cellulose acetate polymers. For gravimetric analysis using ashing techniques, NC Membranes from GVS Life Sciences yield a residue of less than 0.045% of their initial weight. For food and beverage and other microbiological applications, NC membranes are available with a black dye to increase optical contrast. GVS Life Sciences NC Filtration Membrane is available in roll widths from 25 mm (1 in) to 11 cm (31 in), as well as in cut sheets and disks that can be customized to meet your application and size requirements.

Features & Benefits
- High flow rate provides faster filtration
- Lot-to-lot consistency for reliable results every time
- Low extractables ensures clean results
- Passes USP Class VI toxicity testing for use in medical devices
- High protein binding makes this membrane suitable for molecular biology applications

Product Characteristics
- Hydrophilic
- Maximum operating temperature: 356°F (180°C)
- Autoclavable
- Broad sealing compatibility including ultrasonic, heat, radio frequency and insert molding

NC Filtration Membrane
Typical Applications
- Air monitoring
- Aqueous filtration
- Sterility testing
- Gravimetric analysis with ashing technique
- Microbiological and particulate analysis
- Cytology
- HPLC samples
- Food and beverage (black variation only)

Specifications 

Unsupported NC Molecular Transfer (Blotting) Membrane

Typical Applications
- Western blots (proteins)
- Protein and immunoblotting
- Northern and Southern blots (nucleic acids)
- Dot/slot blots
- Radiographic, chromogenic and chemiluminescent detection systems

Specifications 

Supported NC Molecular Transfer (Blotting) Membrane 

This variation of molecular transfer NC is cast on a polyester web to provide increased strength while retaining the binding characteristics of traditional NC. Supported NC may be substituted for unsupported NC without requiring any change of protocol.

Typical Applications
- Western blots (proteins)
- Northern and Southern Blots (nucleic acids)
- Multiple rehybridizations
- Colony/plaque lifts
- Dot/slot blots
- Radiographic detection systems
- Chemiluminescent detection systems
- Biotinylated detection systems

Specifications 

- Sistemi di rilevazione radiografici, cromogenici e chimiluminescenti

Specifiche 

Transfer Membrane molecolare  (Blotting) in NC supportata 

Questa versione per il trasferimento molecolare in NC è fissata su una rete in poliestere per fornire una maggiore resistenza mantenendo le caratteristiche di assorbimento della NC tradizionale. La NC supportata può essere messa al posto di una NC non supportata senza bisogno di alcuna modifica al protocollo.

Applicazioni tipiche
- Western blot (proteine)
- Northern e Southern Blot (acidi nucleici)
- Reibridizzazioni multiple
- Trasferimento di colonie/placche
- Dot/slot blot
- Sistemi di rilevazione radiografici
- Sistemi di rilevazione chimiluminescenti
- Sistemi di rilevazione biotinilati

Specifiche 

Polyvinylidene Difluoride (PVDF) , Molecular Transfer (Blotting) Membrane

GVS Life Sciences Polyvinylidene Difluoride (PVDF) Molecular Transfer Membrane is a naturally hydrophobic unsupported hybridization membrane. It has a high binding capacity and low backgrounds and is ideal for use in protein binding applications such as Western blots, solid phase assays and immunoblotting procedures. PVDF ensures reproducible results with maximum sensitivity. Proteins can be electroblotted from a variety of gel matrices. In addition, PVDF membrane will not degrade, distort or shrink when using a high concentration of methanol for destaining. The exceptional tensile strength allows for easy removal of target bands without concern for the membrane tearing, fracturing or curling. GVS Life Sciences PVDF Transfer Membrane is available in roll widths from 25 mm (1.0 in) to 30 cm (12 in), as well as in sheets and cut disks that can be customized to meet your application and size requirements.

Features & Benefits
- Broad chemical compatibility allows for the use of all commonly used stains
- Low backgrounds ensure the highest sensitivities across a broad range of molecular weights
- High sensitivity provides detection of low-level proteins
- High protein binding capacity (125 μg/cm² for immunoglobulins) allows capture of proteins across a broad range of concentrations
- Strong binding affinity prevents protein from passing through the membrane
- Strict quality control prevents signal variation across membrane lots
- Superior strength prevents distortion and breaking with aggressive handling and processing

Product Characteristics
- Hydrophobic
- Durable
- Typical Applications
- Western blots (protein)
- Protein sequencing
- Amino acid analysis
- Dot /slot blots

Specifications 

Polyvinylidene Fluoride (PVDF) Hydrophilic - PVDF-Plus™

The GVS PVDF Transfer Membrane is a naturally Hydrophilic, unsupported transfer membrane. It has a high binding capacity and low backgrounds and is ideal for use in protein binding applications such as Western blots, solid phase assays and immunoblotting procedures.

Typical Applications
• Western blots
• Immunoblotting
• Solid phase assays
• Amino acid or protein analyses 

Features & Benefits
• Superior strength: Can withstand aggressive handling or be used with automated equipment without breaking or tearing
• Low extractables: Ensures tests will be clean with consistent results
• Exceptional sensitivity: Detects low-level components
• Hydrophilic: For high protein binding
• Lot-to-lot consistency:Quality checks ensure consistent binding for dependable results every time
• BSA protein binding capacity is 125 mg/cm2
• High range of chemical: Resistant to most commonly used chemicals compatible with chemically aggressive solvents 

Exceptional Strength and Chemical Compatibility
GVS PVDF is a naturally Hydrophilic, unsupported transfer membrane. It has a high binding capacity, which prevents protein from passing through the membrane, and a low background that provides for an excellent signal-noise ratio. It also has exceptional tensile strength, preventing it from cracking, tearing, breaking or curling. This membrane also has broad chemical compatibility, which is important when used with common stains such as Amido Black, Colloidal Gold, Coomassie Blue, India Ink and Ponceau-S. GVS PVDF will not degrade, distort or shrink when a high concentration of methanol is used for destaining. Its exceptional strength, high binding capacity and chemical compatibility make GVS PVDF ideal for use in Western blots, immunoblotting, and solid phase assays and plaque lifts. 

Flexibility for OEM Requirements
GVS PVDF Transfer Membrane is available in rolls up to 30 cm (12 inches) wide, as well as sheets and cut disks that can be customized to meet your application and size requirements. Because the GVS PVDF Transfer Membrane is manufactured on-site in GVS facilities, all customization is easy and cost-effective.

Polycarbonate Track Etched (PCTE)

GVS Life Sciences Polycarbonate Track Etched (PCTE) Membrane is made from a thin polycarbonate film with precisely defined pores. It is ideally suited for use in cellular-based filtration assays as well as filtration applications where high purity is required. The membrane is produced through a two-step, proprietary manufacturing process that employs high quality standards. In the first step, polycarbonate film is exposed to ion particles that pass through it. As the ions pass through the film, they create “tracks” where the polymer is damaged. The beamed film is then exposed to a chemical that etches out the tracks creating precise, cylindrical pores. Pore density is controlled by the number of tracks per unit area, and pore size is controlled by varying the temperature, strength and time of exposure to the etching solution. This unique process allows for increased control over pore size and density to ensure the physical properties of each membrane precisely fit your specifications. The resulting membrane is a thin, translucent polycarbonate film with a smooth, flat surface. All particles larger than the pore size are captured on its surface.

To optimize the suitability of PCTE, we offer a variety of products with unique characteristics:
- PVP (polyvinylpyrillidone)-treated for a hydrophilic membrane
- AOX-certified for applications requiring extremely low extractables
- Black-dyed membrane for staining applications
- PVP-free for a hydrophobic membrane

 GVS Life Sciences PCTE Membrane is available in roll widths from 6.35 mm (0.25 in) to 51 cm (20 in) as well as in sheets and cut disks that can be customized to meet your application and size requirements.

 Features & Benefits (of all PCTE variations)
- Absolute pore size and density allows for precise size separation
- Direct thickness and pore size measurements provide accurate characteristics
- Smooth, thin, glass-like surface is suitable for microscopy and cellular applications
- Superior strength allows for aggressive handling
- Low protein binding ensures clean results
- Resists chemical staining to ease microscopic visualization
- Passes USP VI Class toxicity testing for use in medical devices
- Low cytotoxicity offers biocompatibility

Product Characteristics (of all PCTE variations)
- Membrane thickness 5-12 μm
- Maximum operation temperature: 284°F (140°C)
- Autoclavable
- Broad sealing compatibility including ultrasonic, heat, radio frequency and insert molding

PCTE PVP-Treated (Hydrophilic)
Typical Applications
- General filtration
- Removal of red blood cells from plasma
- Flow control of reagents through assays
- Precise filtration and prefiltration
- Fuel testing
- Cytology
- Microscopy

PCTEAOX (Hydrophilic)
Description
GVS Life Sciences PCTE AOX-certified membrane is made from the same thin, microporous polycarbonate film that is the base for our standard PCTE products. AOX-certified membrane is further tested to provide a PCTE membrane with the level of trace volatile, chlorinated organics in the base film at or below 0.03 μg/cm (or 0.15 μg for a 25 mm membrane) by EPA method 8010. It is ideally suited for use in groundwater and wastewater testing to determine the level of organic halides. Halide levels are often regulated and their presence is frequently used as a pollution indicator.

Features & Benefits
- AOX-certified for halide measurement using regulated tests
- Low levels of trace volatile, chlorinated organics eliminates interference with result

Typical Applications
- Groundwater testing
- Wastewater testing
- Food and dairy testing

PCTE Black (Hydrophilic)
Features & Benefits
- Smooth, thin, glass-like surface ensures no microorganisms are caught within the filter structure
- Microorganisms are retained on one plane allowing for easy microscopic visualization
- Lack of staining, negligible autofluorescence and non-specific absorption ensures low backgrounds

Product Characteristics
- Hydrophilic
- Dark gray
- Similar characteristics, except for transparency, as standard PCTE membrane

PCTE PVP-Free (Hydrophobic)
GVS Life Sciences PCTE Membrane is also available as a hydrophobic membrane ideally suited for cellular assays, bioprocessing and diagnostics. This membrane is not treated with PVP, so it retains its natural hydrophobicity. Other characteristics and specifications are similar to those of the PCTE membrane.

Features & Benefits
- Naturally hydrophobic
- Product Characteristics
- Hydrophobic
- Typical Applications
- Cellular assays
- Tissue culture
- Chemotaxis
- Diagnostics

Typical Specifications (all PCTE variations) 

* Reference use only
** Air flow @ 5 psi

Polyester Track Etched (PETE)

GVS Life Sciences PETE Membrane is made from a thin polyester film with a high density of solvent resistance. It is ideal for use in blood assays or general filtration where chemically aggressive solvents may be used. The membrane is produced through a two-step proprietary manufacturing process similar to that of the PCTE membrane. In the first step, polyester film is exposed to ion particles that pass through the film. As the ions pass through the film, they create “tracks” where the polymer is damaged. The beamed film is then exposed to a chemical solution which etches out the tracks creating precise, cylindrical pores. Pore density is controlled by the number of tracks per unit area, and pore size is controlled by varying the temperature, strength and time of exposure to the etching solution. This unique process allows for increased control over pore size and density to ensure the physical properties of each membrane precisely fit your specifications. The resulting membrane is a thin, translucent polyester film with a smooth, flat surface containing pores of controlled diameter and number. The membrane has better solvent resistance than polycarbonate and captures all particles larger than the precisely controlled pore size on its surface.

GVS Life Sciences PETE is available in roll widths from 6.35 mm (0.25 in) to 51 cm (20 in), as well as in sheets and cut disks that can be customized to meet your application and size requirements.

Typical Applications
- General filtration
- Removal of red blood cells from plasma
- Flow control of reagents through assays
- Precise filtration and prefiltration
- Air analysis
- Filtration of aggressive solutions
- Cellular assays and diagnostics
- Trace element analysis

Features & Benefits
- Broad range of chemical compatibility for a wide range of applications
- Direct thickness and pore size measurements ensure accurate characteristics
- Naturally hydrophilic so pre-treatments and wetting agents are not required
- Smooth, thin, glass-like surface for microscopic visualization Low protein binding ensures clean results

Product Characteristics
- Membrane thickness: 10-20 μm
- Naturally hydrophilic
- Maximum operating temperature: 284°F (140°C)
- Autoclavable
- Broad sealing compatibility including ultrasonic, heat, radio frequency and insert molding

Typical Specifications