Protein Array Surface
FAST Slides are glass slides coated with a proprietary nitrocellulose polymer. The polymer binds proteins in a noncovalent, irreversible manner and can be probed using the same method as in traditional blotting.
The 3D surface of a FAST Slide maintains reactivity of proteins and give excellent reproducible results. It is usable with fluorescent, colorimetric, chemiluminescent, or isotopic detection systems and is compatible with microarray scanners and robots.
The most significant advantage of Fast® slides over modified glass surfaces is that the matrix retains arrayed protein in near quantitative fashion for up to a year. This property translates into antibody arrays with unparalleled sensitivity below 1pg/mL in antigen concentration.
These qualities make FAST Slides the most reliable surface for microarray experiments and provide a high level confidence.
FAST Slides are suitable for many types of protein microarrays including protein arrays, reverse phase protein arrays and microspot ELISAs, also known as antibody arrays. There are tremendous advantages to using FAST Slides for immunoassays over traditional ELISAs including less sample required, better sensitivity, linearity and quantitation. A major advantage of FAST Slides technology is that hundreds or thousands of antibodies or samples can be screened simultaneously. Compared to other microarray surfaces, FAST Slides provide superior binding properties, allowing quantitative detection of proteins over four orders of magnitude in concentration.
Characteristics and Advantages
- Binding Properties
- superior protein binding capacity.
- Level of Sensitivity
- highest sensitivity and dynamic range.
- Level of Stability
- excellent long-term stability of printed proteins
- with all detection methodologies and commercially available arraying robots.