Prestained Protein Ladder
The protein ladder is designed for monitoring protein separation during SDS-PAGE, verification of Western blot transfer (PVDF, Nylon or Nitrocellulose membranes) and for the approximate sizing of proteins. Supplied as a ready-to use formulation in gel loading buffer, the ladder requires no heating, dilution or addition of reducing agents. Lot-to-lot variation of apparent molecular weight of prestained proteins is less than 3%. GVS' s protein ladder is available in various colors of purple, yellow, red, orange, green and blue.
Proprietà
1. Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. See the table provided for migration patterns in different electrophoresis conditions.
2. In low-percentage gels (< 10 %), the low-molecular weight proteins in the ladder may migrate with the dye front.
3. Prestained Protein Ladder can be used in Western Blotting with all common membranes: PVDF, Nylon and Nitrocellulose.
4. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100 kDa) proteins.