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        • Flame CoViD -19 Variants Amplification Kit, image 1
        • Flame CoViD -19 Variants Amplification Kit, image 2
        • Flame CoViD -19 Variants Amplification Kit, image 3
        • Flame CoViD -19 Variants Amplification Kit, image 4
        • Flame CoViD -19 Variants Amplification Kit, image 5

        Flame CoViD -19 Variants Amplification Kit

        qPCR Master Kit – CE-IVD

        The kit is able to simultaneously detect the presence of COVID infection of both the “common” strain and the English, South African and Brazilian P2 and other Variant Of Concern with RT-PCR the method in a single “well”. Not Sequencing required!

        This kit is designed with specific primers and Taqman probes for the New Coronavirus ORF1ab and the conservative region of the N gene sequence encoding the nucleocapsid protein of the new coronavirus to diagnose or differentially diagnose whether the tested patient is infected with the new coronavirus, the strain-specific N501Y mutation site and the strain-specific E484K mutation sites.

        It's available now the Flame DELTA VARIANT Reflex Reagent Kit for specific detection of all DELTA Variats. The Flame DELTA VARIANT Reflex Reagent Kit contains specific probes and positive controls and it's just to be added to Flame CoViD-19 Variants qPCR Master kit in second level test in case of sample SARS-CoV-2 positive but N501Y-E484K negative.

        Attachments

        Flame CoViD-19 Variants qPCR Master Kit, CE IVD IFU
        Flame CoViD-19 Variants qPCR Master Kit, CE IVD FLYER
        Flame CoViD-19 Variants qPCR Master Kit, CE IVD MSDS
        Conformity Declaration CE
        Flame DELTA VARIANT Reflex Reagent Kit, R.U.O IFU
        Flame DELTA VARIANT Reflex Reagent Kit, R.U.O FLYER

        Ask for information

        Proprieties

        The mix contains:

        • 2 specific genes: Orf 1ab and N
        • Genomic Internal control
        • Specific sequences for the N501Y Mutation
        • Specific sequences for the E484K Mutation

        4 channels required:

        1. FAM
        2. Cy5
        3. VIC
        4. ROX

        Ability to view data simultaneously and in real-time. “One Step” Workflow.

        Applicable Instruments:

        This kit is suitable for ABI-7500.

        Other devices suitable:
        QuantStudio (ThermoFisher), Applied Biosystems, Bio-Rad (CFX96 Touch 1000, iQ5 iCycler), Agilent (MX-3000, Aria-Dx), MGITech, LighCycler 480II & Z480 (Roche), M2000RT Abbott, Pentabase Gentier48E, SLAN-96S, HIMEDIA INSTA Q96 LA1012, HIMEDIA INSTA Q48 LA1023, MygoPro, Rotor-Gene (6000, 3000, Q6Plex), LineGene 9600, DT-Lite, DT96, DT-Prime thermocycler series and other quantitative fluorescence PCR platforms with FAM, VIC/HEX and Cy5 channels.

        Characteristics

        Box
        48-100 Tests
        Storage
        -20°±5°, protected from light
        Number of freezing and thawing allowed
        not exceed 7 times
        LoD
        500 copies/ml
        Positive agreement
        100%
        Negative agreement
        100%

        Applications

        • Molecular Analysis for CoViD-19 and variants

        Product Variants

        Multiple versions of Flame CoViD -19 Variants Amplification Kit are available.

        Product Variants

        4 available results

        1. FLM0001
          Flame CoViD-19 Variants qPCR Master kit, CE IVD, 48 test
        2. FLM0002
          Flame CoViD-19 Variants qPCR Master kit, CE IVD, 100 test
        3. FLM0003
          Flame DELTA VARIANT Reflex Reagent Kit, R.V.O - 48 test
        4. FLM0004
          Termociclatore Real Time PCR "GENTIER 96E"
        qPCR Master Kit – CE-IVD
        qPCR Master Kit – CE-IVD
        qPCR Master Kit – CE-IVD
        qPCR Master Kit – CE-IVD

        References

        1. SARS-CoV-2-variant-multiple-spike-protein-mutations-United-Kingdom, EuropeanCentreforDiseasePreventionandControl,Stockholm,2020.

        https://www.ecdc.europa.eu/sites/default/files/documents/SARS-CoV-2-variant-multiple-spike-protein-mutations-United-Kingdom.pdf.

        2. ScienceBrief:EmergingSARS-CoV-2Variants,CDC,Jan.28,2021.

        https://www.cdc.gov/coronavirus/2019-ncov/more/science-and-research/scientific-brief-emerging-variants.html

        3. WHO_SARS-CoV-2Variants,DiseaseOutbreakNews,WHO,31December2020.

        https://www.who.int/csr/don/31-december-2020-sars-cov2-variants/en/

        4. EarlytransmissibilityassessmentoftheN501YmutantstrainsofSARS-CoV-2intheUnitedKingdom,LeungKetal.OctobertoNovember2020.

        EuroSurveill.2021Jan;26(1):2002106.doi:10.2807/1560-7917.ES.2020.26.1.2002106.PMID:33413740;PMCID:PMC7791602.

        https://pubmed.ncbi.nlm.nih.gov/33413740/

        5. ReducedneutralizationofSARS-CoV-2B.1.1.7variantbyconvalescentandvaccinesera,

        PiyadaSupasaetal,Cell,2021,ISSN0092-8674,https://doi.org/10.1016/j.cell.2021.02.033

        https://www.sciencedirect.com/science/article/pii/S0092867421002221.

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